Our new Apparent+ tissue clearing technique is the sole approach that delipidates samples without improve in morphology and with negligible impact on structural integrity.
Megatome is actually a vibrating microtome intended to part a wide number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables substantial-throughput tissue sectioning with uniform surface profile, along with nominal tissue hurt and knowledge decline.
Antibodies could acquire months to diffuse by only some millimeters of tissue, that has a steep labeling gradient from area to Main.
Megatome is suitable for precision: the blade vibrates at an increased frequency and bigger amplitude selection than other microtomes, and incorporates a exclusive deflection Management mechanism.
SE uses a rotational electric powered industry to disperse remarkably electromobile molecules (for instance antibodies or surfactant micelles) through a porous sample without having harmful electrically charged structures in the tissue. This allows two-4 day clearing of intact organs,
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eFLASH can be a fast tissue labeling approach that enables for uniform full-organ staining in twenty rounds of labeling.
The SmartSPIM mild sheet microscope presents unparalleled resolution and acquisition pace all over intact tissue volumes, although its modular design lets you upgrade and personalize individual parts.
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Protect avoids the variability of hydrogel embedding and the information reduction from PFA preservation, shielding specimens for many rounds of processing.
Leverage the Distinct+ tissue clearing approach, in megatomi.com conjunction with eFLASH and patented stochastic electrotransport systems, to rapidly distinct and label complete organs. Critical highlights and options include:
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